Effects of three-area laser-assisted zona thinning in 8-cell human embryos on pregnancy outcomes in vitro fertilization.
10.5653/cerm.2018.45.1.25
- Author:
Ju Eun JEONG
1
;
Bo Sun JOO
;
Chang Woon KIM
;
Hwi Gon KIM
;
Jong Kil JOO
;
Kyu Sup LEE
Author Information
1. Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea. kuslee@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Age;
Frozen-thawed embryos;
Laser- assisted hatching;
Multiple thinning;
Pregnancy outcome
- MeSH:
Embryo Transfer;
Embryonic Development;
Embryonic Structures*;
Female;
Fertilization in Vitro*;
Herpes Zoster*;
Humans*;
In Vitro Techniques*;
Pregnancy;
Pregnancy Outcome*;
Pregnancy Rate;
Pregnancy*;
Zona Pellucida
- From:Clinical and Experimental Reproductive Medicine
2018;45(1):25-30
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study conducted a preliminary examination of the effects of three-area laser-assisted zona thinning (LAZT) during the cleavage stage of embryo development on the hatching process in human in vitro fertilization-embryo transfer (IVF-ET) with subjects of advanced female age or frozen-thawed (FT) embryos. METHODS: Eight-cell stage embryos were treated with LAZT in three areas of the zona pellucida at 120° intervals. The control group was embryos without LAZT. Of the 72 consecutive fresh cycles and the 28 FT embryo transfer cycles, the patients in 55 fresh cycles and 17 FT cycles declined LAZT, and those cycles were defined as the control group. RESULTS: In the fresh cycles, the pregnancy rates were similar in the LAZT and control groups. However, in the FT cycles, the pregnancy rate was significantly higher in the LAZT group than in the control group (45.5% in the LAZT group vs. 23.5% in the control group, p < 0.05). CONCLUSION: These results show that multi-area LAZT resulted in significantly improved pregnancy outcomes in human 8-cell embryos compared to controls.
