Effect of high expression of protein tyrosine phosphatase SHP-1 on the tumor cell invasiveness and chemosensitivity of esophageal squamous cell carcinoma
10.3760/cma.j.issn.1006-9801.2018.07.004
- VernacularTitle:蛋白酪氨酸磷酸酶SHP-1高表达对食管鳞状细胞癌细胞侵袭性和化疗敏感性的影响
- Author:
Xinxiong FEI
1
;
Zhongjun JIANG
;
Gangsheng WANG
;
Wenbing HU
;
Xi CHEN
Author Information
1. 435000,鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)普爱院区胸部肿瘤内科
- Keywords:
Esophageal neoplasms;
Protein tyrosine phosphatase;
SHP-1;
Chemotherapy;
Sensitivity
- From:
Cancer Research and Clinic
2018;30(7):447-450,455
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of high expression of protein tyrosine phosphatase SHP-1 on the tumor cell invasiveness and chemosensitivity of esophageal squamous cell carcinoma.Methods EC9706 ceils of esophageal squamous cell carcinoma were divided into three groups:the blank group was not given any treatment;the experimental group used SHP-1 mimic instantaneous vector to transfect cells for 24 h,and used 10 μmol/L cisplatin to treat EC9706 cells for 12 h;the control group selected 10 μmol/L cisplatin to treat EC9706 cells for 12 h.Cell proliferation was detected by using methyl thiazolyl tetrazolium (MTT) assay,and cell invasion was detected by using Transwell chamber.The expression of SHP-1 mRNA was detected by using fluorescence quantitative polymerase chain reaction.The expression level of SHP-1 protein was detected by using Western blot.Results The expression of SHP-1 mRNA in the experimental group (3.42t0.14) was higher than that in the control group and the blank group (1.09±0.13,0.42±0.24,F =9.143,P ＜ 0.05).Compared with the control group and the blank group,the cell growth inhibition rate and the apoptosis index in the experimental group were increased (both P ＜ 0.05),and the differences between any two groups were also statistically significant (all P ＜ 0.05).The cell invasion rate in the experimental group,the control group and the blank group was (6.5±1.3) ％,(18.5±2.5) ％ and (45.2±7.2) ％,respectively,and the difference was statistically significant (F =11.853,P ＜ 0.05).Conclusion High expression of SHP-1 can inhibit the invasion of esophageal squamous cell carcinoma,improve chemosensitivity,promote apoptosis and inhibit cell proliferation,which could lay a theoretical foundation for improving the efficacy of chemotherapy for esophageal squamous cell carcinoma.
