The application of modified capillary immunotyping technique for cryoglobulin identification
10.3760/cma.j.issn.1009-9158.2018.11.009
- VernacularTitle:改良毛细管免疫分型技术在冷球蛋白分型鉴定中的应用
- Author:
Xuzhen QIN
1
;
Pengchang LI
;
Xinxin CAO
;
Jian LI
;
Yajing WANG
;
Li LIU
;
Qian LIU
;
Hang LI
;
Wei SU
Author Information
1. 100730,中国医学科学院北京协和医院检验科
- Keywords:
Cryoglobulins;
Electrophoresis,capillary;
Immunoelectrophoresis
- From:
Chinese Journal of Laboratory Medicine
2018;41(11):832-836
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate the application of modified capillary immunotyping for cryoglobulin qualification .Methods Referred to literature and benchwork experience , a modified capillary immunotyping technique was set up for cryoglobulin identification . Seventy-eight cryoglobulin positive specimens were collected by a standard method in Peking Union Medical College Hospital from November 2016 to July 2017.Thirty-nine samples were identified the type of the cryoglobulin simultaneously by modified capillary immunotyping ( CI ) and immunofixation electrophoresis ( IFE ) .Results Using the modified capillary immunotyping method , the types of cryoglobulin in seventy-eight specimens were identified.The number of cases decreased in the order of Ⅲ, Ⅱ and Ⅰ type of cryoglobulin .The clinical characteristics coincidence with previous reports .The modified CI method had a dramatic advantage in the speed, clarity, and accuracy of results compared with IFE .The ratio of reportable cases between these two methods was 1:0.87.Conclusion The modified capillary immunotyping was an accurate and easy method for cryoglobulin qualification , and feasible for clinical application .
