Genotypes and epidemiological characteristics of Noroviruses in Shenzhen during the winter of 2017
10.3760/cma.j.issn.0254-5101.2018.10.002
- VernacularTitle:2017年深圳地区冬季诺如病毒基因分型与流行病学分析
- Author:
Linlin CHEN
1
;
Yan ZHOU
;
Xiaochen LIN
;
Jing DU
;
Yang LIU
;
Yi WANG
;
Hongjun LI
Author Information
1. 650118 昆明,中国医学科学院北京协和医学院 医学生物学研究所,云南省重大传染病疫苗研发重点实验室
- Keywords:
Norovirus;
Genotype;
Phylogenetic tree;
Homology analysis
- From:
Chinese Journal of Microbiology and Immunology
2018;38(10):725-730
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the genotypes, epidemiological characteristics and homology of Noroviruses (NoV) circulating in Shenzhen in the winter of 2017. Methods RT-PCR was performed using Nov-specific primers after extracting viral genome from 313 fecal samples. Positive RT-PCR products were then sequenced. Phylogenetic trees were constructed based upon the gene sequences of isolated and reference NoV strains using Mega 4. 1 and Clustal W software. Results There were 26 NoV-positive samples and all belonged to G Ⅱ. 4 subtype. These strains shared high homologies with G Ⅱ. 4 ( KY407156), G Ⅱ. 4 (KY580757) and GⅡ. 4 (KX372682). Phylogenetic analysis also suggested that 88. 46％ of them had a lower homology with the NoV strains isolated in the same area in recent years and 46. 15％ were different from the epidemic strains in other provinces of China. Conclusion NoV GⅡ. 4 was the epidemic strain in Shenzhen during the winter of 2017. More attention should be paid to it from the local public health authori-ties considering its owned characteristics in epidemic and homology.
