Role of Lactobacillus paracasei prtP gene in regulating mouse intestinal immunity
10. 3760/cma. j. issn. 0254-5101. 2018. 05. 003
- VernacularTitle:prtP基因对副干酪乳杆菌调节小鼠肠道免疫作用的影响
- Author:
Chen ZHANG
1
;
Zhao LIU
;
Zhihong YAO
;
Jian HU
;
Bo LI
;
Wenqi FAN
;
Huiqing XU
Author Information
1. 225127,扬州大学食品科学与工程学院
- Keywords:
Lactobacillus paracasei;
prtP gene;
Intestinal immunity
- From:
Chinese Journal of Microbiology and Immunology
2018;38(5):337-344
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of serine protease encoding gene prtP carried by Lactoba-cillus paracasei (Lp) in regulating mouse intestinal immunity. Methods Female BALB/c mice were ran-domly divided into seven groups and respectively administered with PBS, medium (5×108CFU/ml) and high dose (1×109CFU/ml) of Lp, Lp△prtP and ReLp△prtP for 21 consecutive days by intragastric ga-vage. Parameters including immune organ index, splenic lymphocyte transformation rate, energy metabolism in celiac macrophages, phagocytic ability of celiac macrophages and cytokines were detected. Quantitative changes in CD11c+CD80+cells in culture supernatants of splenic mononuclear cells were analyzed by flow cytometry. Results Compared with Lp△prtP, Lp could significantly promote the natural transformation of splenic lymphocytes, increase the level of energy metabolism in celiac macrophages, enhance the phagocytic ability of celiac macrophages, and up-regulate the expression of IFN-α, IL-10 and IFN-γ and the percentage of CD11c+CD80+cells. Conclusion Serine protease encoding gene prtP in Lp can regulate the mucosal im-mune system in mice.
