Clinical data and next-generation sequencing results analysis of central nervous system infection with listeria
10.3760/cma.j.issn.1006-7876.2018.06.009
- VernacularTitle:中枢神经系统李斯特菌感染患者的临床和脑脊液二代测序结果分析
- Author:
Xiaojuan WANG
1
;
Hongzhi GUAN
;
Ke WEI
;
Lingzhi QIN
;
Honglong WU
;
Yongjun LI
;
Wei LI
Author Information
1. 河南省人民医院神经内科
- Keywords:
Next-generation sequencing;
Meningitis;
Encephalitis;
Cerebrospinal fluid;
Listeria
- From:
Chinese Journal of Neurology
2018;51(6):451-455
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the potential value of next-generation sequencing ( NGS) as a diagnostic method for listeria infection in central nervous system ( CNS ) . Methods To identify the potentially pathogenic microorganisms ( PPMs) of the five patients with CNS infection in the Department of Neurology, People's Hospital of Zhengzhou University from June 2017 to November 2017, blood or cerebrospinal fluid ( CSF ) was detected not only using common laboratory tests , including routine , biochemical, cytologic, culture and Gram-staining methods, etc, but also using the BGISEQ-100 sequencing platform to identify the PPMs of CSF .Concomitantly , we collected concurrent clinical data , then performed a comprehensive analysis of their clinical , laboratory , and auxiliary examination data .Results Of the five cases (male :female: 1:4, age ranges: 26 -61 years), the disease mainly occurred in summer.Three patients received immunosuppressants treatment before infection , and three patients had gastrointestinal syndrome in the prodromal period .Infection of CNS led to fever , headache and meningeal irritation sign in all the patients.The medical imaging examinations showed the invasion of meninges , brainstem, and the periventricular gray matter.The cell count of CSF was more than 500 ×106/L.NGS techniques showed listeria genome sequence ranged from 57 to 2611, and the coverage of listeria varied from 0.23％ to 14.00％, and PCR results supported the existence of listeria .Conclusion NGS is beneficial to make the diagnosis of listeria infection in CNS , and can help guide early treatment .
