Fsp27 gene regulate the activity of primary hepatic stellate cells in vitro
10.3760/cma.j.issn.1007-631X.2018.07.019
- VernacularTitle:Fsp27基因抑制原代肝星状细胞活化与活性的实验研究
- Author:
Qigang XU
1
;
Bo XU
;
Fuxiang YU
Author Information
1. 温州医科大学附属第一医院肝胆外科
- Keywords:
Hepatic stellate cells;
Gene expression;
Fsp27
- From:
Chinese Journal of General Surgery
2018;33(7):592-595
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fat-specific protein 27 (Fsp27) gene on primary hepatic stellate cells (HSCs) in vitro.Methods Rt-PCR was used to detect the expression of Fsp27 gene in primary and activated HSCs.The lentivirus carrying Fsp27 was constructed and transfected into primary HSCs.Effect of Fsp27 gene on proliferation of HSCs was tested by CCK-8 colorimetry.Detection of HSCs α-actin (oα-SMA) expression was tested by Western blotting.Effect of Fsp27 gene on expression of fibrosisrelated genes in HSCs were tested by Real-time quantitative PCR.Results The difference of Fsp27 gene between primary HSCs and activiated HSCs was significant (Fsp27 mRNA:2 700 ± 500,5 ± 2,t =124.27,P ＜ 0.05).After coculture for 72h,the proliferation and activation of HSCs was inhibited by Fsp27 (48 h:31.5±1.3,6.1±0.1,t=17.35,P＜0.05;72h:36.2±2.2,6.2±0.1,t=21.94,P＜0.05).Fsp27 can enhance the expression of MMP-2 gene (0.48 ± 0.02,0.85 ± 0.14,1.63 ± 0.21,F =41.56,P ＜ 0.05) and down-regulate the expression of TGF-[1 gene in activiated HSCs (3.2 ± 0.17,2.0 ± 0.3,1.7 ± 0.2,F =21.52,P ＜ 0.05).Conclusion Fsp27 gene can inhibit the proliferation and activation of primary HSCs.Fsp27 can regulate the expression of fibrosis related gene.Fsp27 may play an important role in maintenance the quiescent phenotype of HSCs.
