Expression of cytidine triphosphate synthetase 1 in pancreatic cancer cell lines and its clinical significance
10.3760/cma.j.issn.1007-631X.2018.02.014
- VernacularTitle:三磷酸胞苷合成酶1在胰腺癌细胞株的表达及其临床意义
- Author:
Ning PU
1
;
Shanshan GAO
;
Guochao ZHAO
;
Yadong XU
;
Yang LYU
;
Abulimiti NUERXIATI·
;
Wenhui LOU
;
Wenchuan WU
Author Information
1. 复旦大学附属中山医院普外科
- Keywords:
Cytosine nucleotide;
Pancreatic neoplasms;
Prognosis
- From:
Chinese Journal of General Surgery
2018;33(2):149-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cytidine triphosphate synthetase 1 (CTPS1) expression in pancreatic cancer cell lines and its impaction on prognosis.Methods The GEO profiles database was searched to collect clinical data of 39 patients of pancreatic ductal adenocarcinoma.Kaplan-Meier curve was used to analyze the relationship between the patients' prognosis and the expression of CTPS1.qPCR and Western blot were applied to detect the expression of CTPS1 in 4 pancreatic cancer cell lines.Results The mRNA expression of CTPS1 in Sw1990,BxPC-3,Panc1,and MIA-Capa2 was 1.00) ± 0.20,2.92±0.95,4.29±0.14,and 2.26±0.33 (t=-16.19,-11.45,-8.09,-13.12,P＜0.05) when compared to the normal pancreatic ductal epithelial cell line HPDE of 7.70 ± 0.72.The relative protein expression of CTPS1 in pancreatic cancer cell lines Sw1990,BxPC-3,Panc1,and MIA-Capa2 was 0.40 ± 0.06,0.20±0.09,0.68±0.11,and0.48±0.06 (t=-8.97,-10.5,-4.39,-7.88,P＜0.05) when compared to HPDE of 1.09 ±0.12.The overall survival (OS) in patients with higher CTPS1 expression than adjacent tissue was 28 months (95％ CI:9.2-46.8 months),and that with lower CTPS1 expression was 13 months (95％ CI:8.2-17.8 month),(x2 =4.02,P＜0.05).Conclusions CTPS1 in the pancreatic cancer cell lines are of low expression,and the level is positively related to patients' survival time.
