Establishment of HPLC fingerprints of Kangfuxin Liquid and determintion of six constituents
10.3969/j.issn.1001-1528.2018.03.021
- VernacularTitle:康复新液HPLC指纹图谱建立及6种成分测定
- Author:
Na L(U)
1
;
Jun-Chi WANG
;
Li-Hua CHEN
;
Lian-Gang SHEN
;
Guang-Zhi LI
;
Jian-Yong SI
Author Information
1. 中国医学科学院北京协和医学院药用植物研究所
- Keywords:
Kangfuxin Liquid;
HPLC fingerprints;
uracil;
hypoxanthine;
xanthine;
inosine;
protocatechuic acid;
Cyclo (Gly-Tyr)
- From:
Chinese Traditional Patent Medicine
2018;40(3):613-617
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the HPLC fingerprints of Kangfuxin Liquid (extract of Periplaneta americana L.) and to determine the contents of six constituents.METHODS The analysis of this drug was performed on a TOSOH TSK-GEL ODS column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.07％ acetic acid) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS There were twenty-four common peaks in the fingerprints of ten batches of samples (Ⅰ-Ⅹ) with the similarities of 0.932-0.993 (except for sample Ⅰ).Uracil,hypoxanthine,xanthine,inosine,protocatechuic acid and Cyclo (Gly-Tyr) showed good linear relationships within the ranges of 3.460-173.0,3.960-198.0,3.596-179.8,1.338-66.9,3.672-183.6 and 3.552-177.6 μg/mL,whose average recoveries (RSDS) were99.8％ (2.65％),98.0％ (2.55％),99.7％ (1.59％),100.7％ (2.80％),102.0％ (2.09％) and 99.6％ (1.88％),respectively.CONCLUSION This accurate,stable and simple method can be used for the quality control of Kangfuxin Liquid.
