Simultaneous determination of five constituents in Simotang Oral Liquid by HPLC
10.3969/j.issn.1001-1528.2018.01.022
- VernacularTitle:HPLC法同时测定四磨汤口服液中5种成分
- Author:
Wei-Dong ZHANG
1
;
Guo-Liang DAI
;
Yong-Tao BAI
;
Li-Jing ZHU
;
Wen JING
;
Wen-Zheng JU
Author Information
1. 南京中医药大学附属医院临床药理科
- Keywords:
Simotang Oral Liquid;
synephrine;
norisoboldine;
naringin;
hesperidin;
neohesperidin;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(1):114-117
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of five constituents in Simotang Oral Liquid (Aucklandiae Radix,Aurantii Fructus,Arecae Semen,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent Zorbax C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 283 nm.RESULTS Synephrine,norisoboldine,naringin,hesperidin and neohesperidin showed good linear relationships within the ranges of 5.8-185.6 μg/mL (r =0.999 9),0.829-26.52 μg/mL (r =1.000 0),9.775-312.8 μg/mL (r =1.000 0),0.594-19 μg/mL (r =0.999 5) and 5.2-166.4 μg/mL (r =1.000 0),whose average recoveries were 98.93％,98.95％,102.57％,99.67％ and 103.43％ with the RSDs of 1.85％,1.27％,0.52％,0.89％ and 0.43％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the rapid quality control of Simotang Oral Liquid.
