Effect of application of pulsed radiofrequency to dorsal root ganglia on activation of spinal astrocytes in a rat model of neuropathic pain
10.3760/cma.j.issn.0254-1416.2018.06.014
- VernacularTitle:背根神经节脉冲射频对神经病理性痛大鼠脊髓星形胶质细胞活化的影响
- Author:
Jingzhi LIU
1
;
Kemei SHI
;
Xiaojuan WANG
;
Wenting MA
;
Quanbo LI
;
Huixing WANG
;
Baosen ZHENG
;
Guolin WANG
Author Information
1. 300211,天津医科大学第二医院疼痛治疗中心
- Keywords:
Catheter ablation;
Ganglia,spinal;
Neuralgia;
Spinal cord;
Astrocytes
- From:
Chinese Journal of Anesthesiology
2018;38(6):691-694
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of application of pulsed radiofrequency to dorsal root ganglia on activation of spinal astrocytes in a rat model of neuropathic pain (NP).Methods Eighty male Sprague-Dawley rats,aged 6-8 weeks,weighing 200-250 g,were divided into 4 groups (n=20 each) using a random number table method:sham operation group (group Sham),group NP,pulsed radiofrequency group (PRF group) and sham pulsed radiofrequency group (group SPRF).NP was induced by chronic constriction injury (CCI).The mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured at 1 day before CCI and 1,7,14 and 21 days after CCI.Four rats were sacrificed at 1 day before CCI and 14 and 21 days after CCI,and the L4.6 segments of the spinal cord were harvested to detect the expression of glial fibrillary acidic protein (GFAP) and interleukin-1beta (IL-1β) by Western blot.Results Compared with group Sham,the MWT was significantly decreased and the TWL was shortened at each time point after CCI,and the expression of GFAP and IL-1β was up-regulated at 14 and 21 days after CCI in NP,PRF and SPRF groups (P＜0.05).Compared with group NP,the MWT was significantly increased and the TWL was prolonged at 14 and 21 days after CCI (P＜0.05),and the expression of GFAP and IL-1β was down-regulated at 14 and 21 days after CCI in group PRF (P＜0.05),and no significant change was found in the parameters mentioned above in group SPRF (P＞0.05).Conclusion The mechanism by which pulsed radiofrequency reduces NP is probably related to inhibiting spinal astrocyte activation in rats.
