Application of 18 F-FDG microPET/CT in the screening of cerebral ischemia reperfusion models
10.3760/cma.j.issn.2095-2848.2018.11.004
- VernacularTitle:18F-FDG microPET/CT在脑缺血再灌注模型筛选中的应用
- Author:
Yimeng PENG
1
;
Chunyin ZHANG
;
Lu YU
;
Hua TAN
;
Qiang YOU
Author Information
1. 西南医科大学附属医院核医学科
- Keywords:
Brain ischemia;
Reperfusion;
Positron-emission tomography;
Tomography;
X-ray computed;
Deoxyglucose;
Rats
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2018;38(11):726-730
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of 18F-fluorodeoxyglucose (FDG) microPET/CT in the screening of cerebral ischemia reperfusion ( CIR) models. Methods The suture-occluded method was used to establish CIR rat models with reversible middle cerebral artery embolism. After that only awake rats whose Zea-Longa scores were 1-4 were selected for the following experiments, and 18 male SD rats were selected. Garcia scale with 18 points was used to evaluate the neurological function of rats at 2 and 24 h post-operation. At the same time points, 18 F-FDG was injected into caudal vein after anesthesia and micro-PET/CT scan was conducted at 40 min post-injection. Visual and semi-quantitative analyses were adopted to analyze the images. The autopsy and HE staining were performed on accidentally dead rats. The other alive rats were sacrificed after microPET/CT scan at 24 h post-operation, and their brain tissues were taken out quickly to detect the infarction by triphenyl tetrazolium chloride ( TTC) staining. The pathological results were taken as the gold criteria. Fisher exact test was used to compare the difference of accuracy for diagno-sing CIR models between neurological function score ( NFS) and microPET/CT. Results According to the pathology, there were 11 CIR models, 4 with subarachnoid hemorrhage ( SAH) , 3 with SAH and cerebral hemorrhage. Between 8-12 h post-operation, 4 rats died accidentally. At 2 h post-operation, the diagnostic accuracies of NFS and microPET/CT were 11/18 and 15/18 (P<0.05). At 24 h post-operation, the diag-nostic accuracies of NFS and microPET/CT were 11/14 and 14/14, respectively, no statistical difference was observed( P>0.05) . Conclusion 18 F-FDG microPET/CT is better than NFS in screening CIR models in early stage.
