Applicability Study on QAMS for Multi-component Content Determination of Lonicerae Japonicae Caulis
10.3969/j.issn.1005-5304.2018.11.017
- VernacularTitle:一测多评法用于忍冬藤多成分含量测定的适用性研究
- Author:
Wei TIAN
1
;
Ya-Qin ZHEN
;
Xin-Guo WANG
;
Yu-Rou TIAN
;
Jun-Shan LI
;
Li-Ying NIU
Author Information
1. 河北中医学院
- Keywords:
QAMS;
relative correction factors;
chlorogenic acid;
Lonicerae Japonicae Caulis;
quality evaluation
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(11):77-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a QAMS method for content determination of six compositions (chlorogenic acid, caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and loganin) from Lonicerae Japonicae Caulis; To verify the feasibility and applicability of this method in quality control of Lonicerae Japonicae Caulis. Methods Chlorogenic acid was set as internal reference substance. The HPLC analysis was performed on a Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase consisted of acetonitrile and 0.4% phosphoric acid solution in gradient elution manner at a flow rate of 1 mL/min. The column temperature was maintained at 35 ℃, and the detection wavelength was set at 327 nm for chlorogenic acid, caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and 236 nm for loganin. Results The relative correction factors of caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and loganin were established; there was no obvious difference between calculated value of QAMS and measured value of external standard method. Conclusion The quality control mode of QAMS can be used for multi-index synchronization quality evaluation of the six compositions from Lonicerae Japonicae Caulis.
