Effects of angiotensin Ⅱ on protein kinase Cε and Cα expression in hepatic stellate cells
10.3760/cma.j.issn.1008-1372.2018.07.010
- VernacularTitle:血管紧张素Ⅱ对肝星状细胞蛋白激酶Cε、Cα表达的影响
- Author:
Wenyan QIN
1
;
Chunfang XU
;
Qi LIN
;
Zhangzhang SONG
;
Lingyun SONG
;
Guojun LI
Author Information
1. 315040 宁波市鄞州区第二医院消化科;215006 苏州大学附属第一医院消化科
- Keywords:
Angiotensin Ⅱ/PD;
Hepatic stellate cells/DE/EN;
Protein kinase C-alpha/ME;
Protein kinase C-epsilon/ME;
Cell proliferation
- From:
Journal of Chinese Physician
2018;20(7):995-998
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of angiotensin Ⅱ on protein kinase Cε (PKCε) and protein kinase Cα (PKCα) expression in hepatic stellate cells.Methods Hepatic stellate cell (HSC)-T6 cells were treated with different concentrations of angiotensin Ⅱ and the proliferation of HSC-T6 cells was detected by methyl thiazolyl tetrazolium (MTT) assay.The expression of PKCε and PKCα was detected by immunofluorescence staining.PKCε and PKCα mRNA levels was detected by real time polymerase chain reaction (PCR).Results Angiotensin Ⅱ concentrated the proliferation of HSC-T6 cells and the level of hydroxyproline (F =25.321,13.283,P ＜ 0.001) and showed a dose-dependent effect.With the increase of angiotensin Ⅱ concentration,PKCε significantly increased and translocated in the cell membrane;PKCα increased significantly,especially in transplanted membrane and cytoplasm (F =21.387,19.431,P ＜0.01),and showed obvious dose effect.Meanwhile,Angiotensin Ⅱ increased the expression of PKCε and PKCα,and induced cell proliferation by up-regulating PKCε and PKCα mRNA levels (F =13.279,15.174,P ＜ 0.05).Conclusions Angiotensin Ⅱ can up-regulate the expression of collagen in hepatic stellate cells in a dose-dependent manner,increase the expression of protein kinase Cε and Cα,and promote the proliferation of hepatic stellate cells.
