Determination of Three Components in Xylaria Nigripes Powder by HPLC

VernacularTitle:HPLC法测定乌灵菌粉中3种成分的含量
Author: Long LI ¹ ; Shanbo MA ; Jin WANG ; Shan MIU ; Ying CHEN ; Aidong WEN ; Xiaopeng SHI
Author Information

1. 空军军医大学西京医院药剂科西安710032;陕西中医药大学药学院
Keywords: Xylaria nigripes; HPLC; Determination; Adenosine; Genistein; 5-Hydroxymellein
From: China Pharmacist 2018;21(3):527-529
CountryChina
Language:Chinese
Abstract: Objective:To establish a HPLC method for the simultaneous determination of adenosine,genistein and 5-hydroxymel-lein in Xylarin nigripes powder.Methods:The chromatographic separation was carried out on a Pntulips@BP-C18column (250 mm× 4.6 mm,5 μm) at 35℃. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution at a flow rate of 1.0 ml ·min-1,and the detection wavelength was 254 nm. Results:The linear range of adenosine was 0.162-1.62 μg(r=0.999 2). The average recovery was 98.12% and the RSD was 1.32% (n=6). The linear range of genistein was 0.02-0.2 μg(r=0.999 9). The average recovery was 98.89% and the RSD was 1.10% (n =6). The linear range of 5-hydroxymellein was 0.022-0.22 μg(r =0.999 9). The average recovery was 99.87% and the RSD was 0.98% (n=6). Conclusion:The method can be used to determine the contents of adenosine,genistein and 5-hydroxymellein,which provides the basis for the quality control and the further research of Xylaria nigripes.