Simultaneows Determination of Strychnine and Brucine in Shiyifang Vinum by LC-MS/MS

VernacularTitle:UPLC-MS/MS同时测定十一方药酒中的马钱子碱与士的宁的含量
Author: Lizhi WEI ¹ ; Shihua LU
Author Information

1. 广西医科大学第二附属医院南宁530007
Keywords: Strychnine; Brucine; Shiyifang vinum; LC-MS/MS; Content determination
From: China Pharmacist 2018;21(2):348-350
CountryChina
Language:Chinese
Abstract: Objective:To develop a determination method for strychnine and brucine in Shiyifang vinum by LC-MS/MS. Meth-ods:The samples were separated on an Agilent Zorbax SB-C18column(2.1 mm×100 mm,3.5 μm). The mobile phase was metha-nol-10 mmol·L-1ammonium formate solution (adjusting pH to 3.0 with methane acid)(25: 75), and the flow rate was 0.2 ml· min-1. The eletrospray ionization( ESI) with positive source of the mass spectrometer and multiple reaction monitor( MRM) mode were used to detect strychnine(m/z 395.2→243.8;m/z 395.2→212.8)and brucine(m/z 335.2→183.9;m/z 335.2→155.9). Re-sults:The linear range of strychnine was 0.242-7.733 μg·ml-1,and that of brucine was 0.302-9.661 μg·ml-1. The average re-covery was 100.1% (RSD=0.8%,n=6) and 99.4% (RSD=1.4%,n=6),respectively. Conclusion:The method is simple and accurate,which can be used to determine the contents of strychnine and brucine in Shiyifang vinum.