Effects of Shengji Huayu Ointment on Total IL-1β,Collagen TypeⅠand Collagen TypeⅢin Granulation Tissue of Mice in Wound Repair Period
- VernacularTitle:生肌化瘀膏对小鼠创面修复期肉芽组织总IL-1β和Ⅰ、Ⅲ型胶原的影响
- Author:
Yuxuan ZHANG
1
;
Lingling XU
;
Bin LI
;
Fulun LI
Author Information
1. 上海中医药大学附属岳阳中西医结合医院药剂科 上海200437
- Keywords:
Shengji Huayu ointment;
IL-1β;
Collagen type I;
Collagen type Ⅲ
- From:
China Pharmacist
2018;21(2):246-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of promoting wound healing of Shengji Huayu ointment. Methods: Db/db mice were divided into 5 groups according to the randomized block design: three Shengji Huayu ointment groups respectively at high (15 g·kg-1),middle (10 g·kg-1) and low(5 g·kg-1) dose,the positive control group(Beifuji) and the model control group, and balb/c mice were used in the normal control group,and each group was with 10 mice. The model of full-thickness skin defect in mouse skin was used,and after HE staining,IL-1β and collagen type I and type III in granulation tissue were observed by immunohis-tochemistry and image analysis. Results:HE staining showed that the wound healing was obvious in the high dose group and the mid-dle dose group. The expression of IL-1 β in each group was not obvious. The levels of collagen type I and type III in the three Shengji Huayu ointment groups were higher than those in the model control group (P<0.05).Conclusion: Shengji Huayu ointment can pro-mote wound healing and reduce scar formation in mice.
