Pleurotus citrinopileatus alleviates alcohol-induced fatty liver via upregulating AMPK signaling
- Author:
Ben-Wen CUI
1
;
Yu ZHANG
;
Xia LI
;
Kai-Li XIA
;
Min JIANG
;
Yan-Ling WU
;
Ji-Xing NAN
;
Li-Hua LIAN
Author Information
1. Key Laboratory for Natural Resource of Changbai Mountain&Functional Molecules
- Keywords:
Pleurotus citrinopileatus;
alcoholic hepatosteatosis;
AMPK;
P2x7R
- From:
Chinese Journal of Pharmacology and Toxicology
2018;32(4):276-276
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE The current study was designed to investigate the anti-steatosis effect of Pleurotus citrinopileatus extract (PC) and the underlying mechanism in vivo and in vitro. METHODS Acute and chronic alcoholic hepatosteatosis murine models and ethanol-treated HepG2 cells were applied. RESULTS In vitro,the anti-steatosis effect of PC was further confirmed via Nile red staining in HepG2 cells treated with ethanol.Both of acute and chronic alcohol-induced mice hepatosteatosis model,PC decreased serum aminotransferase and triglyceride accumulation. Upregulated sterol-regulatory element binding protein1(Srebp1),purinergic ligand-gated ion channel 7receptor(P2X7R)and downregulated sirtuin1 (SIRT1), adenosine 5′-monophosphate (AMP)-activated protein kinase α (AMPKα) caused by acute and chronic alcohol intake were modulated by PC.In ethanol-exposed HepG2 cells,PC reduced lipid accumulation in a concentration-dependent manner and exhibited superior ability in controlling lipid accumulation compared with metformin. CONCLUSION PC could abolish hepatic lipid accumulation through regulating SIRT1-AMPKα signaling in acute and chronic alcohol-induced hepatic steatosis.
