Identification of potential protein targets of atractylodin against cholangiocarcinoma
- Author:
Wanna CHAIJAROENKUL
1
;
Kanawut KOTAWONG
;
Sittiruk ROYTRAKUL
;
Narumon PHAONAKROP
;
Kesara NA-BANGCHANG
Author Information
1. Graduate Program in Bioclinical Sciences
- Keywords:
atractylodin;
Atracylodes lancea;
cholangiocarcinoma;
LC-MS/MS
- From:
Chinese Journal of Pharmacology and Toxicology
2018;32(4):262-262
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To identify potential cell signaling pathways and protein targets of the active compound isolated from Atracylodes lancea "atractylodin" in cholangiocarcinoma, using proteomics approach. METHODS The holangiocar- cinoma cell line was exposed with atractylodin for 3 and 6 h and the proteins from both intra- and extra- cellular components were extracted. The extract proteins were separated by SDS-PAGE and digested with trypsin.The LC-MS/MS was applied to identify proteins. Signaling pathways and protein expression were analyzed by MASCOT and STITCH software.RESULTS A total of 4,323 and 4,318 proteins were identified from intra-and extracellular components,respectively. Six intracellular proteins were linked with the signaling pathways (apoptosis, cell cycle control, and PI3K-AKT).Four extracellular proteins were linked with the signaling pathways(NF-κB and PI3K-AKT). CONCLUSION All these proteins will further study to confirm the link to the anticholangiocarcinoma ac-tivity of actractylodin.
