Effect of baicalein on reversal of multidrug resistance in MCF-7/MX cells in vitro and its mechanisms
10.3969/j.issn.1001-1978.2018.06.024
- VernacularTitle:黄芩素对BCRP介导的人乳腺癌MCF-7/MX细胞多药耐药的逆转作用
- Author:
Nai-Jie FU
1
;
Chang WANG
;
Di-Ying ZHU
;
Lin-Hong LIU
;
Hui-Feng ZHANG
;
Rui-Zan SHI
;
Ming-Sheng ZHANG
Author Information
1. 山西医科大学基础医学院药理学教研室
- Keywords:
baicalein;
multidrug resistance;
breast cancer resistance protein;
mitoxantrone;
p-p38;
NF-κB
- From:
Chinese Pharmacological Bulletin
2018;34(6):862-866
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of baicalein on the reversal of multidrug resistance ( MDR) media-ted by breast cancer resistance protein ( BCRP) in hu-man breast cancer MCF-7/MX cells, and explore the possible mechanisms. Methods MTT assay was per-formed to determine the cytotoxicity of baicalein and susceptibility of chemotherapeutic drugs. The protein expression levels of BCRP, p-p38 MAPK and NF-κB p65 were determined by Western blot. Results MCF-7/MX cells were not only resistant to MX but cross-re-sistant to 5-FU and DDP, and the resistance index was 70. 45, 6. 68 and 21. 47, respectively. 2. 5, 5μmol· L-1 of baicalein could increase the sensitivity to above chemotherapeutic agents and decrease the expression levels of BCRP, p-p38 MAPK and NF-κB p65 in MCF-7/MX cells. Conclusion Baicalein can effec-tively reverse MDR of MCF-7/MX by down-regulating BCRP expression through p38/MAPK and NF-κB path-ways.
