Effect of Shuanglong formula on no-reflow in rats with myocardial ischemia/reperfusion
10.3969/j.issn.1001-1978.2018.04.027
- VernacularTitle:双龙方对大鼠心肌缺血/再灌注后无复流影响的研究
- Author:
Feng-Jie LI
1
;
Yi-Kui LI
;
Ping HE
;
Xin-Mei HAO
;
Chao-Hua LI
;
Le ZHAO
;
Wei HAO
;
Cheng-Hao YU
;
Jin-Yan ZHANG
Author Information
1. 中国中医科学院西苑医院基础医学研究所
- Keywords:
Shuanglong formula;
rat;
myocardial is-chemia/reperfusion;
myocardial infarct;
no-reflow;
ul-trasound cardiogram;
myocardial contrast echocardio-graphy
- From:
Chinese Pharmacological Bulletin
2018;34(4):583-588
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of Shuanglong formula(SLF) on no-reflow in rats with myocardial is-chemia/reperfusion (I/R). Methods The rats were divided into five groups, namely, sham group, I/R group,SLF(5,2.5,1.25 g·kg-1)group. Treatment group received SLF decoction by gavage once a day for five days,while other groups were offered drinking wa-ter by gavage once a day for five days. The rats in I/R group and SLF-pretreated group were induced by iga-tion of left anterior descending coronary artery,and the rats were subjected to ischemia for 4h followed by reperfusion. Sham operation group did not undergo oc-clusion of the coronary artery. After 4 hours' reperfu-sion, real-time myocardial contrast echocardiography was used to monitor regional blood perfusion and cardi-ac functions. Blood was collected from the abdominal aorta and the serum was separated, and the levels of cTnT, CRP, CK and LDH were measured. The myo-cardial no-reflow area and infarction area were assessed by thioflavin S and nitrotetrazolium blue chloride, re-spectively. Results The SLF-pretreated group exhibi-ted significant reductions in the infarct area and no-re-flow area compared with I/R group(P <0.01 or P <0.05). In SLF-pretreated groups, β, A and A·β significantly increased as compared to those in I/R group. The LV anterior wall systolic and diastolic thicknesses (LVAW d/s) were significantly improved in SLF-pretreated group compared with those in I/R group. The LV internal diameter in systole (LVID s) and the LV volume in systole(LV s) were significantly reduced in SLF-pretreated group compared with those in I/R group. The EF, FS and SV were significantly improved in SLF-pretreated group compared with those in I/R group. The comparison between SLF-pretreated group and I/R group showed no significant difference in LDH, CK, cTnT, and CRP levels. Conclusion Shuanglong formula minimizes the sizes of myocardial infarct area and no-reflow area,improving regional my-ocardial blood flow and cardiac function.
