Effects of gastrodin against SH-SY5Y cell autophagy induced by methamphetamine and underlying mechanisms
10.3969/j.issn.1001-1978.2018.04.026
- VernacularTitle:天麻素在甲基苯丙胺诱导SH-SY5Y细胞自噬中的作用及机制研究
- Author:
Gen-Meng YANG
1
;
Juan LI
;
Shi-Jun HONG
;
Feng-Lin XUE
;
Yong-Wang HE
;
Yi-Qing ZHOU
;
Xiao-Feng ZENG
;
Li-Hua LI
Author Information
1. 昆明医科大学法医学院
- Keywords:
methamphetamine;
SH-SY5Y cells;
gast-rodin;
autophagy;
neurotoxicity;
signaling pathway
- From:
Chinese Pharmacological Bulletin
2018;34(4):577-583
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of gastrodin on SH-SY5Y cell autophagy induced by methamphet-amine (METH) and the underlying mechanisms. Methods SY5Y cells were treated by METH with the concentration of 0.5,1.0,1.5,2.0,2.5,3.0 mmol·L-1for 24 h. The morphological changes were ob-served by microscopy,the expression of LC3-Ⅱ,Bec-lin-1,Akt,p-Akt,mTOR and p-mTOR were detected by Western blot. Gastrodin was added to the medium 1 h before METH treatment. Results The SY 5 Y cells were morphologically featured by shrinkage and den-drite disruption after exposed to METH(0~3 mmol· L-1),and autophagic vacuoles occurred in cytoplasm. The expression of LC3-Ⅱ increased over METH dose. Confocal results showed that LC3-Ⅱsignificantly in-creased in METH group as compared with control, while decreased in METH+ Gastrodin group. The ex-pression levels of LC3-Ⅱand Beclin-1 significantly in-creased (P<0.01) in METH group, p-mTOR and p-Akt decreased, and mTOR and Akt showed no signifi-cant difference as compared with control. However, the gastrodin could decrease the expression of LC3-Ⅱand Beclin-1 and increase the expression of mTOR,p-mTOR,Akt and p-Akt as compared with METH-trea-ted groups. Conclusions METH can induce SY5Y cells autophagy. The protective effect of gastrodin a-gainst METH-induced autophag may be related to gast-rodin regulation mTOR and Akt signaling pathway.
