Effect of adenosine on autophagy and proliferation of hepatocellular carcinoma cells
10.3969/j.issn.1001-1978.2018.04.014
- VernacularTitle:腺苷对肝癌细胞自噬和增殖的作用
- Author:
Ze-Jin PU
1
;
Xiao-Tao ZHOU
;
Guo-Ping LI
;
Hao-Lian ZHAN
;
Yi-Tian GUO
;
Meng-Qi XIANG
;
Li-Xuan LIU
;
Hui TAN
;
Ling-Fei WU
Author Information
1. 汕头大学医学院第二附属医院消化内科
- Keywords:
adenosine;
autophagy;
multi-drug resist-ance;
hepatocellular carcinoma;
cell proliferation;
tumor therapy
- From:
Chinese Pharmacological Bulletin
2018;34(4):508-512
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of adenosine on the autophagy and proliferation of hepatocellular carcinoma cells, and improve the curative effect of a-denosine on hepatocellular carcinoma. Methods HepG2 cells were incubated with adenosine, CCK-8 method was used to study the changes of cell prolifera-tion,Western blot was used to study the expression of LC3-Ⅱ and LC3-Ⅰ, and MDC staining was used to observe the number of autophagosomes. Results HepG2 cells were incubated with adenosine(1.0~4.0 mmol·L-1) for 48 h,the proliferation of HepG2 cells were detected at the different time points (12,24,48 h),and the result showed the proliferation was signifi-cantly inhibited by adenosine (P < 0.01). HepG2 cells were incubated with adenosine (0.2,0.5,1.0, 2.0,4.0 mmol·L-1) for 24 h,the ratio of LC3-Ⅱ/LC3-Ⅰ decreased significantly in low concentration of adenosine group (0.2, 0.5 mmol·L-1, P <0.05;1.0 mmol·L-1,P<0.01),and the ratio of LC3-Ⅱ/LC3-Ⅰ increased significantly in higher concentration of adenosine group (4.0 mmol·L-1, P <0.05). HepG2 cells were incubated with adenosine(1.0 mmol·L-1) for 24 h, the ratio of LC3-Ⅱ/LC3-Ⅰ de-creased significantly at 6,12 and 24 h detecting point, the number of autophagosomes were reduced, the low-est ratio of LC3-Ⅱ/LC3-Ⅰ and autophagosomes were observed at 12 h detecting point(P<0.01). Conclu-sions Adenosine inhibits the proliferation of hepato-cellular carcinoma cells,the low concentration of aden-osine inhibits the autophagy,while the high concentra-tion of adenosine increases the autophagy, which is of great significance to reduce multi-drug resistance and improve the therapeutic effect of anti-hepatoma drugs.
