Study on role of TIGIT signal in Th1/Th2 balance in Schistosoma japonicum-infected mice
10.16250/j.32.1374.2017233
- VernacularTitle:TIGIT信号在日本血吸虫感染小鼠Th1/Th2反应平衡中的作用研究
- Author:
Li-Na ZHANG
1
;
Xiao-Fan WANG
;
Qian-Qian QI
;
Li-Yang DONG
;
Lei XU
;
Ya-Nan PU
;
Chuan WEI
;
Ji-Feng ZHU
;
Sha ZHOU
;
Ya-Lin LI
;
Feng LIU
;
Xiao-Jun CHEN
;
Chuan SU
Author Information
1. 南京医科大学病原生物系
- Keywords:
Schistosoma japonicum;
TIGIT;
Th1/Th2 balance;
Flow cytometry
- From:
Chinese Journal of Schistosomiasis Control
2018;30(2):136-139,144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of TIGIT signal in Th1/Th2 balance in the process of Schistosoma japonicum in-fection.Methods Male C57BL/6 mice were infected with cercariae of S.japonicum,and normal uninfected mice served as the controls.The percentages of TIGIT+cells,Ki67+CD3+CD4+TIGIT+cells,Ki67+CD3+CD4+TIGIT-cells,IFN-γ+CD3+CD4+TIGIT+cells,IFN-γ+CD3+CD4+TIGIT- cells,IL-4+CD3+CD4+TIGIT+cells and IL-4+CD3+CD4+TIGIT- cells were evaluated in mouse spleen by flow cytometry.Results The proportion of TIGIT+CD4+T cells was higher in the spleen of S.japonicum-infected mice than in the normal uninfected mice(29.30%±0.70% vs.3.09%±0.50%;t=8.834,P<0.01).However,no significant differ-ence in the percentages of TIGIT+CD8+T cells between the infection group and normal controls(3.61% ± 0.26% vs. 3.58% ± 0.16%;t=0.108,P>0.05),and no significant difference was detected in the percentages of TIGIT+cells in non-T cells be-tween the infection group and controls(1.86%±0.19% vs.1.37%±0.17%;t=1.931,P>0.05).In addition,the proportion of Ki67 in the TIGIT+cells was higher than that in the TIGIT-cells(17.03%±0.64% vs.6.59%±0.37%;t=14.09,P<0.01).The Th2/Th1 ratio was higher in the TIGIT+CD4+T cells than in the TIGIT-CD4+T cells(39.28%±3.75% vs.11.79%±1.64%;t=6.721,P<0.01).Conclusion The TIGIT signaling may be involved in the development of Th2 responses in the mice infected with S.japonicum.
