Effect of neurotrophic factor-3 on the proliferation and apoptosis of bone marrow mesenchymal stem cells and mechanisms
10.3760/cma.j.issn.1674-6554.2018.01.003
- VernacularTitle:神经营养因子-3对骨髓间充质干细胞增殖凋亡的影响及机制
- Author:
Hui WANG
1
;
Hetao BIAN
;
Jing SHI
;
Cuiping SI
;
Qian LIU
;
Yafei ZHOU
;
Ling WU
;
Zhongrui YAN
Author Information
1. 山东省医学科学院附属济宁市第一人民医院神经内科
- Keywords:
Bone marrow mesenchymal stem cells;
Neurotrophin-3;
CCAAT/enhancer binding proteins β;
Neuronal;
Apoptosis
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2018;27(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of neurotrophin-3(NT-3)on the proliferation and apoptosis of bone marrow mesenchymal stem cells(BMSCs)in rats and possible mechanisms. Methods The NT-3 overexpression and lentiviral transfection of BMSCs were co-cultured with neuronal cells respectively and then they were divided into overexpression control group,NT-3 transfection group and shRNA-NT-3 transfection group(NT-3 silencing group).MTT assay was used to detect the cell culture for 24 h,48 h and 72 h. Cell cycle and apoptosis were detected by flow cytometry for 48 h. Real-time quantitative PCR was used to detect the expression of C/EBPβmRNA.The expression of C/EBPβprotein was detected by Western blot. Results MTT results showed that the proliferation ability of BMSCs in the NT-3 overexpres-sion group was significantly higher than that in the control group(0.650±0.042,0.826±0.074)at 48 h and 72 h(P<0.05).Compared with the control group(P<0.05),the cell cycle and apoptosis of BMSCs in NT-3 silencing group were significantly decreased at 48 h and 72 h(P<0.05). The results of 48 h cell cycle and apoptosis showed that the percentage of G1 phase in BMSCs was decreased,G2 and S were increased and the apoptosis was decreased. The percentage of G1 phase in G2-S phase and the increase of apoptosis were in-creased in NT-3 silencing group. The results of Western Blot showed that C/EBPβ mRNA and protein levels were significantly up-regulated in BMSCs of NT-3 overexpression group and significantly decreased in NT-3 silencing group(P<0.05).Conclusion NT-3 may promote the expression of C/EBP beta and affect the ex-pression of its downstream target genes,which can inhibit the apoptosis of BMSCs cells.
