Establishment and application of a PCR detection method for Tupaia (tree shrew)paramyxovirus(TPMV)
10.3969/j.issn.1671-7856.2018.05.018
- VernacularTitle:树鼩副粘病毒(TPMV)PCR 方法的建立及初步应用
- Author:
Shujing WANG
1
;
Rui FU
;
Xiaobo LI
;
Ji WANG
;
Zhengming HE
;
Bingfei YUE
Author Information
1. 中国食品药品检定研究院实验动物资源研究所
- Keywords:
Tupaia(tree shrew);
paramyxovirus;
PCR;
detection
- From:
Chinese Journal of Comparative Medicine
2018;28(5):100-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and preliminarily apply an effective PCR assay for detection of Tupaia(tree shrew)paramyxovirus(TPMV). Methods Using TPMV genomic DNA from NCBI GenBank, bases 8231 to 8720 were synthesized and inserted into a plasmid as a positive standard. One primer pair was designed based on this sequence. In total,60 respiratory swabs and 12 lung tissues from the tree shrews were tested in this PCR assay. Results A PCR method for detection of TPMV was successfully established,with high specificity and sensitivity of 11.5 × 10 -5μg/mL. PCR result testing 60 respiratory swabs and 12 lung tissues were negative. Conclusions PCR for detecting TPMV has good specificity and high sensitivity and can be used for conventional tree shrew paramyxovirus detection.
