Efficacy and safety of the removal of intestinal endotoxin in the blood by affinity adsorption materials
10.3969/j.issn.1005-4847.2018.01.008
- VernacularTitle:亲和吸附材料特异性清除肠源性内毒素有效性和安全性探讨
- Author:
Yongping XIANG
1
;
Hui CUI
;
Dan LIU
;
Jie YU
;
Lidong ZHOU
;
Jianming YANG
;
Aibing LIU
Author Information
1. 锦州医科大学武警总医院研究培养基地
- Keywords:
endotoxin;
intestinal endotoxin;
hemoperfusion;
affinity adsorption material;
dogs
- From:
Acta Laboratorium Animalis Scientia Sinica
2018;26(1):52-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficacy and safety of the affinity adsorption material for removal of intes-tinal endotoxin in the blood. Metheds To establish a canine model of intestine-derived endotoxemia by ligation and perfo-ration of the appendix. Hemoperfusion was performed to treat the endotoxemia in model dogs. In the experiment,we moni-tored vital signs and detect the content of endotoxin and blood physiological and biochemical indexes at different time points. Results The endotoxin content was(0.49 ± 0.22),(0.034 ± 0.00)Eu/mL after hemoperfusion for 1 and 2 hours,compared with that before the beginning of perfusion(7.25 ± 1.18)Eu/mL,showing a significant difference(P <0.05). After treatment for 2 hours,the average clearance rate was 99.52%. White blood cells,red blood cells,hemoglo-bin,platelets,total protein,albumin,globulin,alanine aminotransferase,aspartate aminotransferase,urea and creatinine index were significantly decreased after perfusion(P < 0.05). The index values were in a normal range and vital signs were stable during the perfusion. Conclusions The affinity adsorption material developed by our team can effectively re-move endotoxin in the blood of experimental dogs and has good blood compatibility.
