Effects of Sel1L on properties of bone marrow derived dendritic cells
10.3969/j.issn.1000-484X.2018.05.017
- VernacularTitle:Sel1L对小鼠骨髓源树突状细胞的影响
- Author:
Jie XU
1
;
Nan-Nan YAN
;
Chuan-Xiang ZHAO
;
Ci LI
;
Yi WU
;
Teng-Fei XIAO
;
Feng-Wei GAO
;
Wen-Hui ZHOU
;
Qi-Xiang SHAO
;
Qiao-Ming LONG
;
Sheng XIA
Author Information
1. 江苏大学医学院免疫学与免疫检验学教研室
- Keywords:
Sel1L;
Dendritic cell;
Endoplasmic reticulum stress;
Co-stimulatory molecule;
CD4+T cell
- From:
Chinese Journal of Immunology
2018;34(5):727-731
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of suppress or enhancer of lin-12-like(Sel1L) on differentiation and function of bone marrow-derived dendritic cells.Methods:To generate conditional knockout mice by the Cre-Loxp recombination system.ELISA and Real-time fluorescence quantitative PCR(RT-PCR) was used for analyzing the protein levels and mRNA levels of IL-6/IL-12 in BMDCs.The protein levels of Sel1L in BMDCs were detected by Western bolt.The expression of CFSE,CD80,CD86,MHC-Ⅰ,MHC-Ⅱon BMDCs and the capability in priming OVA specific CD4+T cells proliferation were analyzed by the flow cytometry.Results:The deficiency of Sel1L decreases the proliferation of DCs during its differentiation,up-regulates the secretion of IL-6,IL-12 and the expression of MHC-Ⅰ.Notably,Sel1L-null DCs was failed to up-regulate MHC-Ⅱexpression and dramatically impaired their ability to prime OVA323-339specific CD4+T cell.Conclusion:The deletion of Sel1L can reduce the proliferation of BMDCs and down-regulate its ability in priming the proliferation of OVA specific CD4+T cells.
