Evaluation of cytotoxicity of stearoyl gemcitabine-loaded PLGA nanoparticles to Lewis lung cancer cells
10.3969/j.issn.1000-484X.2018.02.013
- VernacularTitle:吉西他滨PLGA纳米颗粒对小鼠Lewis肺癌细胞增殖抑制效应的实验研究
- Author:
Lan WU
1
;
Yuan-Qiang ZHENG
;
Zhe CHEN
;
Yu-Chen JIA
;
Feng DING
;
Zheng-Rong CUI
;
Yan-Chun SHI
Author Information
1. 内蒙古医科大学自治区分子生物学重点实验室
- Keywords:
Gemcitabine;
Nanoparticle;
PLGA;
Lewis lung cancer cells
- From:
Chinese Journal of Immunology
2018;34(2):223-226,238
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anti-proliferation effect of 4-(N)-stearoyl gemcitabine-loaded poly(lactic-co-glycolic) acid nanoparticles(GemC18-PLGA-NPs) on Lewis lung cancer cells(LLC) in vitro.Methods: Lewis cells were incubated with GemC18-PLGA-NPs,free GemC18,gemcitabine HCl(GemHCl) or GemC18-free blank nanoparticles(PLGA-NPs) respectively and cell viability was determined using an MTT assay after 24,48 or 72 h of incubation.The apoptosis rate after 48 and 72 h of incubation were measured by flow cytometry.Results:GemC18-PLGA-NPs,GemC18,and GemHCl all significantly inhibited the growth of LLC cells, and the survival rate of GemHCl group was lowest,GemC18-PLGA-NPs group had the highest survival rate.The cell survival rate of GemC18-PLGA-NPs after 72 h was significantly higher than that of GemHCl (P<0.05) at the concentration of 1 μmol/L,indicating that it had a significant drug release effect.PLGA-NPs group produced trifle inhibition on the Lewis cells without correlation to time or concentration.Conclusion:GemC18-PLGA-NPs have significant anti-proliferation effect on mouse Lewis lung cancer cells in vitro.
