Diff erential expression analysis of microRNA gene chip in coronary particles and peripheral blood particles in patients with acute myocardial infarction with thrombus aspiration
10.3969/j.issn.1004-8812.2018.01.009
- VernacularTitle:急性心肌梗死行血栓抽吸患者冠状动脉内微粒与外周血微粒中microRNA基因芯片的差异性表达分析
- Author:
Ning-Xin WEN
1
;
Ling-Yun ZU
;
Gui-Song WANG
;
Jie NIU
;
Yong-Zhen ZHANG
;
Jiang-Li HAN
;
Ming CUI
;
Wei GAO
Author Information
1. 100191 北京,北京大学第三医院心内科, 卫生部心血管分子生物学与调节肽重点实验室, 分子心血管学教育部重点实验室, 心血管受体研究北京市重点实验室
- Keywords:
Acute myocardial infarction;
Thrombus aspiration;
Microparticles;
Gene chip;
microRNA
- From:
Chinese Journal of Interventional Cardiology
2018;26(1):41-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diff erential expression of microRNA in microparticles from coronary blood and peripheral blood in patients with acute myocardial infarction, and to provide clues for further study on the role of myocardial in the pathogenesis of myocardial infarction. Methods Coronary and peripheral blood samples were collected from patients with acute myocardial infarction undergoing thrombus aspiration. Microparticles from coronary and peripheral blood samples were isolated by centrifugation and gene chips were used to sequence the microRNA from the microparticles in the two groups. The diff erences in microRNA expression were identifi ed between two groups and the function of these microRNA were analyzed. Results There were signifi cant diff erences between the microRNA in the microparticles from the coronary blood and peripheral blood in patients with acute myocardial infarction. By constructing expression profi les, 307 diff erentially expressed microRNA were found, with 221 of them were up regulated and 86 of them were down regulated. Conclusion There is signifi cant diff erence between the expression of microRNA in microparticles from the coronary blood and the peripheral blood of patients with acute myocardial infarction forty nine of them are closely related to cardiovascular disease, which can be used as the target of further research.
