The influence of sample components on "Coffee Ring" DNA test
10.13618/j.issn.1001-5728.2018.02.005
- VernacularTitle:样品成份对"咖啡环"法DNA检测的影响
- Author:
Fei ZHAN
1
;
Guisen ZHAO
;
Yaqin LI
;
Jiong CHEN
;
Wan GUO
Author Information
1. 河南科技大学法医学院
- Keywords:
forensic biological evidence;
DNA;
"coffee ring" effect;
NaCl;
surfactant;
pH
- From:
Chinese Journal of Forensic Medicine
2018;33(2):134-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influences of drop components on the"coffee ring" test of DNA. Methods The DNA-EB drops containing SDS, NaCl, etc, were evaporated on glass slides. After evaporation, the fluorescent Deposition Pattern (DP) and Integrated Optical Density (IOD) was acquired with a gel imaging system. The dose-effect relationship was analyzed. Result When the non-DNA components concentration is low, the DP was still characteristiced by peripheral rings, subtle component-specific differences, such as tree-ring like structure and radial crack, existed. At high concentrations, DP was various, which may be ring + scattered dots (NaCl), central spot + small weak ring (SDS), concentric/tree-ring (TritonX-100) or ring + spot (H+), et al. Non-DNA components had little effect on IOD(0.5~2 times). Conclusion Non-DNA components in DNA-EB drops influences both the DP and IOD, but rough estimation of DNA concentrations is still effective. Moreover, analyzing the DP can provide more informations about sample purity and residue.
