Effect of toosendanin on invasion and migration of human ovarian cancer cells
10.3969/j.issn.1000-4718.2018.01.012
- VernacularTitle:川楝素对人卵巢癌细胞侵袭和迁移的影响
- Author:
Yu-Ying LI
1
;
Ke-Na ZHANG
;
Jin-Wei CAI
;
Jiang-Tao LIN
;
Xi-Ying SHAO
Author Information
1. 衢州职业技术学院医学院
- Keywords:
Toosendanin;
Ovarian cancer;
Cell invasion;
Cell migration;
Epithelial-mesenchymal transition;
Nuclear factor-κB
- From:
Chinese Journal of Pathophysiology
2018;34(1):70-74
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of toosendanin(TSN)on invasion and migration abilities of human ovarian cancer cells and the related mechanism.METHODS:The human ovarian cancer cell lines CAVO-3 and SKVO-3 were treated with TSN at different concentrations.The cell viabilty at 12,24,48,72 and 96 h after TSN treatment was measured by CCK-8 assay.Scratch wound healing assay and Transwell assay were employed to measure the invasion and migration abilities of CAVO-3 cells.The protein expression of nuclear factor-κB(NF-κB)p65,E-cadherin,N-cadherin,vimentin and Snail was determined by Western blot.RESULTS:TSN significantly inhibited the viability of CAVO-3 and SKVO-3 cells(P<0.05 ).Compared with control group ,the migration and invasion abilities of CAVO-3 cells in TSN group decreased significantly(P<0.05).In addition,the expression of NF-κB p65 and E-cadherin protein increased no-tably,followed with N-cadherin,vimentin and Snail protein decreased significantly(P<0.05).However,the inhibitor of NF-κB BAY11-7082 reversed the impact above.Compared with TSN group ,the migration and invasion abilities in TSN +BAY11-7082 group increased significantly(P<0.05).The protein expression of E-cadherin also decreased notably ,fol-lowed with the protein expression of N-cadherin,vimentin and Snail increased significantly(P<0.05).CONCLUSION:TSN inhibits the invasion and migration abilities of human ovarian cancer cells ,which is related to the inhibition of epitheli-al-mesenchymal transition process mediated by NF-κB/Snail signaling pathway.
