Comparative study on Development of Mouse Embryos in Conventional Medium versus Vero Cell Coculture.
- Author:
You Sung LEE
1
;
Chang Ho LEE
;
Hee Jung GO
;
Ky Suk LEE
;
Chul Hee RHEU
;
Jong Duk KIM
Author Information
1. Department of Obstetrics and Gynecology, College of medicine, Chonbuk National University, Chonju, Korea.
- Publication Type:Comparative Study ; Original Article
- Keywords:
IVF-20;
Vero cell;
Coculture
- MeSH:
Animals;
Blastocyst;
Cercopithecus aethiops;
Coculture Techniques*;
Embryonic Development;
Embryonic Structures*;
Epithelial Cells;
Female;
Humans;
Kidney;
Male;
Mice*;
Mice, Inbred ICR;
Morula;
Oviducts;
Pregnancy;
Vero Cells*
- From:Korean Journal of Obstetrics and Gynecology
2002;45(6):1026-1032
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To evaluate mouse embryos development in conventional medium IVF-20 versus vero cell coculture. METHODS: Female ICR mice aged 6 to 8 weeks, were stimulated with 5IU PMSG and 48 hours later were injected 5IU of hCG, then female and male mice were mated. At 48 hour post-hCG injection, oviducts were dissected out and 2-cell embryos were flushed. The 2-cell embryos were cultured in IVF-20 media or media containing vero cell (African green monkey kidney epithelial cell lines) for 120 hours. Coculture techniques have been applied in mouse 2-cell embryos culture used vero cell lines. RESULTS: 1. After 48 hours culture, 60.7% and 55.7% of 2 cell embryos developed to 4 cell and morulae stage, respectively, in IVF-20 culture medium, but significantly less embryos developed to 4 cell (47.6%, p<0.05) and momlae (42.9%, p<0.05) in vero cell coculture. 2. After 72 hours culture, 51.6% of 2 cell embryos developed to blastocyst and expanded blastocyst in IVF-20 culture medium, but significantly less embryos developed to blastocyst and expanded blastocyst (25.9%, p<0.01) in vero cell coculture. 3. After 96 hours culture, 37.7% and 32.6% of 2 cell embryos similar developed to expanded blastocyst and hatching in IVF-20 culture medium and vero cell coculture, respectively. 4. After 120 hours culture, 36.9% and 37.4% of 2 cell embryos similar developed to expanded blastocyst and hatching in IVF-20 culture medium and vero cell coculture, respectively. CONCLUSION: There was no difference of embryo development rates between the two culture groups. IVF-20 medium alone gives a benefit to the viability of an embryo compared with a vero cell coculture.
