Retinal ganglion cell-conditioned medium alters the differentiation of rat retinal progenitor cells
10.13389/j.cnki.rao.2018.0007
- VernacularTitle:视网膜神经节细胞无血清上清培养液诱导大鼠视网膜干细胞的分化
- Author:
Min DAI
1
;
Qing ZHANG
;
Zhi-Kun ZHENG
;
Wei SHEN
Author Information
1. 云南省第二人民医院眼科云南省眼科研究所云南省眼科疾病防治研究重点实验室
- Keywords:
retinal stem cells;
retinal ganglion cells;
retinal ganglion cell-conditioned medium;
Nestin;
Thy-1
- From:
Recent Advances in Ophthalmology
2018;38(1):35-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of retinal ganglion cell-conditioned medium on the differentiation of retinal stem cells.Methods Rettial stem cells and retinal ganglion cells were isolated from rats,and immunofluorescence staining was applied to identify rat retinal stem cells and retinal ganglion cells with Nestin and Thy-1 antibody,respectively.Retinal stem cells were cultured in presence or absence of retinal ganglion cell-conditioned medium for 72 h,followed by detection of Nestin,PAX6,Thy-1 and Bin-3 gene expression in retinal stem cells by qPCR.Results isolated retinal stem cells were Nestin positive,and retinal ganglion cells were Thy-1 positive,indicating the success of isolation.Compared to retinal stem cells cultured without ganglion cellconditioned medium,ones cultured with ganglion-conditioned medium had significantly downregulated expression of Nestin and PAX6 (both P ＜ 0.000 1),and markedly upregulated expression of Thy-1 and Brn-3 (both P ＜ 0.05).Conclusion Retinal ganglion cell-conditioned medium can induce the differentiation of retinal progenitor cells into retinal ganglion-like cells.
