TNF-α promotes the proliferation of colon cancer cells by activating the Wnt/β-catenin pathway
10.7652/jdyxb201804012
- VernacularTitle:TNF-α通过Wnt/β-catenin信号通路促进结肠癌细胞的增殖
- Author:
Jun-Hui YU
1
;
Xue-Jun SUN
;
Jian-Bao ZHENG
;
Wei WANG
;
Kui YANG
;
Guang-Bing WEI
Author Information
1. 西安交通大学第一附属医院普通外科
- Keywords:
colorectal cancer;
TNF-α;
proliferation;
Wnt/β-catenin pathway
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2018;39(4):504-508
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of treatment with TNF-α on growth ability of colon cancer cells and its possible mechanism.Methods Human colon cancer HT-29 cells were treated with TNF-α(10 nmol/L). The effect of TNF-αon the proliferative ability was examined by cell growth curve assay and MTT assay,respectively. Cell cycle was detected by flow cytometry.Protein levels ofβ-catenin,c-myc and cyclinD1 were detected by Western blot.We also observed the effect of the Wnt/β-catenin pathway blockage by XAV9 3 9 on TNF-α's promoting prolif-eration of colon cancer cells and proteins related to the Wnt/β-catenin pathway.Results Upon treatment with TNF-α,the proliferative ability of HT-29 cells was enhanced (all P＜0.05),G0/G1 phase cell ratio was decreased (P＜0.05),and S phase cell ratio was increased (P＜0.05).The protein levels ofβ-catenin,c-myc and cyclinD1 were increased in TNF-α-treated HT-29 cells (all P＜0.05).XAV939 treatment resulted in a significant inhibition of cell proliferation ability (all P＜0.05)as well as the protein levels ofβ-catenin,c-myc and cyclinD1 (all P＜0.05) in the TNF-α-treated HT29 cells.Conclusion TNF-αmay be involved in the occurrence and development of colon cancer by activating the Wnt/β-catenin pathway and promoting the proliferation of colon cancer cells.
