Impacts of LASP-1 on osteogenic moving behavior and distribution of cell cycle of osteoblasts derived from different soue
10.3969/j.issn.1006-5725.2018.09.007
- VernacularTitle:LASP-1对不同来源成骨细胞的运动、成骨功能及细胞周期分布的影响
- Author:
Fang JIA
1
;
Chunhua LAI
;
Zhen ZHOU
;
Yan GAO
;
Zehong GUO
;
Shulan XU
Author Information
1. 南方医科大学口腔医院 广州510082
- Keywords:
LASP-1;
non tumor derived;
tumor derived;
osteogenesis;
cell cycle
- From:
The Journal of Practical Medicine
2018;34(9):1428-1434
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impacts of LASP-1 on the behavior and cell proliferation ability of tumor derived osteoblast-like cells and non tumor derived osteoblasts. Methods QRT-PCR and Western blot were performed to detect LASP-1 expressions in mouse bone marrow mesenchymal stem cells(BMMSC),human osteoblasts (hOB) and human osteosarcoma cell (MG-63). With the construction of recombinant vector and the transfection of shLASP-1,the expression changes in both LASP-1 and osteocalcin(OCN),and the cell number of cell-cycle phase distribution were analyzed. Results With up-or down-regulation of LASP-1,osteocalcin(OCN) expression,cell proliferation ability and the cell number of G2/M phase in non tumor cells(BMMSC,hOB)had no obvious changes. However,as for the tumor cells(MG-63),with an increased expression of LASP-1,the expression of OCN,cell proliferation number and the cell number at G2/M phase changed. There was no significant effect on the moving ability of each cell. Conclusions LASP-1 has no significant effect on the osteogenic proper-ties,cell proliferation ability and cell cycle distribution of non tumor derived cells,but has a regulatory effect on tumor derived cells(MG-63).
