Profiling of AKT inhibitors on tumor infiltrating T lymphocytes in patients with liver metastasis of colorec-tal cancer
10.3969/j.issn.1006-5725.2018.03.004
- VernacularTitle:AKT抑制剂对结直肠癌肝转移患者肿瘤浸润T淋巴细胞生物学活性的影响
- Author:
Benling XU
1
;
Jinxue ZHOU
;
Long YUAN
;
Guangyu CHEN
;
Lu HAN
;
Peng QIN
;
Quanli GAO
Author Information
1. 郑州大学附属肿瘤医院 生物免疫治疗中心 郑州450008
- Keywords:
colorectal cancer;
tumor infiltrating t lymphocytes;
central memory t cells;
akt inhibitor
- From:
The Journal of Practical Medicine
2018;34(3):352-356
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of AKT inhibitors on tumor infiltrating T lymphocytes (TIL)in patients with liver metastasis of colorectal cancer.Methods The tumor tissues from the patients with liv-er metastasis of colorectal cancer in Department of General Surgery,The Affiliated Cancer Hospital of Zhengzhou University from January 2016 to December 2016 were collected.TIL and tumor cells were isolated by percoll densi-ty gradient centrifugation. The profiling of AKT inhibitors on TIL were analyzed by flow cytometry. Results AKT inhibition enhances the expansion of TIL with memory cell without affects its proliferation,also the cells obtained under AKT inhibitor with IL-2 showed higher frequency of IFN-γproducing cells than IL-2. Conclusion Add AKT inhibitors in TIL cultivation system can strengthen the proliferation of central memory T cells,and does not affect the number of CD8+T cells.This might be developed for cell-based immunotherapy of cancer.
