p38 MAPK pathway promotes IL-6 and IL-8 secretion of synovial fluid mesenchymal stem cells from temporomandibular joint inflamed by IL-1β
10.3969/j.issn.1001-3733.2018.02.015
- VernacularTitle:IL-1β激活p38 MAPK通路促进人颞下颌关节滑液间充质干细胞分泌IL-6和IL-8
- Author:
Wenjing LIU
1
;
Yangpeng SUN
;
Youhua ZHENG
;
Zhoutao WANG
;
Zhiguang ZHANG
Author Information
1. 南方医科大学口腔医院修复科
- Keywords:
Temporomandibular joint;
Synovial fluid mesenchymal stem cells;
Cytokines;
Mitogen activated protein kinase(MAPK)
- From:
Journal of Practical Stomatology
2018;34(2):215-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the role of p38 MAPK pathway in IL-1β induced inflammatory secretory properties mesenchymal stem cells(SFMSCs) from human synovial fluid of temporomandibular joint. Methods: In vitro cultured hSFMSCs were divided into control group and IL-1β treated group(10 ng/ml IL-1β for 2 h), the activation of p38 MAPK was examined with western blot. Then, the gene expression and the secretion of IL-6 and IL-8 in control group, IL-1β treated group and p38 MAPK inhibitor pre-treated group(pretreatment with SB-203580 10 μmol/L prior to the addition of 10 ng/ml IL-1β) were examined by RT-PCR and cytometric bead array(CBA). Results: It was observed that the protein level of p-p38 MAPK, the secretion of IL-8 and IL-6 were increased in IL-1β treated group, and suppressed in p38 MAPK inhibitor pre-treated group. Conclusion: p38 MAPK pathway of SFMSCs might play a role in IL-6 and IL-8 secretion by SFMSCs in inflammatory environment.
