The effects of long non-coding RNA linc-01135 on the osteogenic differentiation capability of human inflammatory PDLSCs under static mechanical strain loading
10.3969/j.issn.1001-3733.2018.02.010
- VernacularTitle:长链非编码RNA linc-01135对静态牵张力作用下人炎症牙周膜干细胞成骨分化的影响
- Author:
Wanhua ZOU
1
;
Wen QIN
;
Yuerong XU
;
Zaixiu QIN
;
Jia LIU
;
Zuolin JIN
Author Information
1. 710032 西安,军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病临床医学研究中心,第四军医大学口腔医院正畸科
- Keywords:
Long non-coding RNA;
Periodontal ligament stem cells(PDLSCs);
Static mechanical strain(SMS);
Osteogenic differentiation
- From:
Journal of Practical Stomatology
2018;34(2):193-197
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of long non-coding RNA linc-01135 on the osteogenic differentiation of inflammatory PDLSCs(P-PDLSCs) under 12％ static mechanical strain loading. Methods: Cells were isolated and cultured from the healthy and periodontitis samples respectively to obtain healthy PDLSCs(H-PDLSCs) and P-PDLSCs. RT-PCR were used to identify the expression level of linc-01135. Lentiviruses were used to upregulate and downregulate the expression of linc-01135, and the osteogenesis gene expression were analyzed by RT-PCR and the osteogenesis differentiation capability were evaluated by alizarin red staining. Results: linc-01135 expression in P-PDLSCs was lower than that in H-PDLSCs. When the expression of linc-01135 was upregulated in P-PDLSCs before 12％ SMS loading, the expression level of RUNX2, ALP, OPG was significantly increased. In contrast, the expression of RUNX2, ALP, OPG was significantly decreased when the expression of linc-01135 was suppressed. Alizarin red staining proved the same trend. Conclusion: linc-01135 can promote the osteogenic differentiation capability of P-PDLSCs under 12％SMS loading.
