Expression of Adhesion Molecules CD44 and ICAM-1 in Mycoplasma Pneumoniae Infected BALB/c Mice and the Related Mechanisms
10.3969/j.issn.1671-7414.2018.01.022
- VernacularTitle:黏附分子CD44和ICAM-1在肺炎支原体感染BALB/c小鼠体内表达及相关机制
- Author:
Shi-Qun DENG
1
;
Shi-Yuan ZHAO
;
Bang-Xian ZHANG
Author Information
1. 广西南宁市西乡塘区石埠卫生院检验科
- Keywords:
mycoplasma pneumonia (MP);
adhesion molecule;
CD44;
ICAM-1
- From:
Journal of Modern Laboratory Medicine
2018;33(1):91-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of adhesion molecules CD44 and ICAM-1 in BALB/c mice infected with Mycoplasma pneumoniae (MP) and elucidate the relationship between MP and infection.Methods BALB/c mouse MP model was established by MP nasal instillation,and the pathological changes of lung in MP mice were observed.The expression levels of adhesion molecules CD44 and ICAM-1 in serum and bronchoalveolar lavage fluid of mice infected with 5,8,15,20 and 30d MP were determined by ELISA.Results Compared with the model group,MP infected BALB/c mice after lung inflammation was significantly up-regulated MP,the expression in BALB/c mice infected with the serum and bronchoalveolar layage fluid in CD44 and adhesion molecule ICAM 1 (t5 d serum CD44 =53.64 ng/L,t5 d BALF CD44 =144.3 ng/L;t5 d serum ICAM-1 =73.72 ng/L,t5 d BALF ICAM-1 =165.06 ng/L,all P＜ 0.000;t8 d serum CD44 =40.86 ng/L,t8 d BALF CD44 =21.31 ng/L;t8 d serum ICAM-1 =30.57 ng/L,t8 d BALF ICAM-1 =19.61 ng/L,all P＜0.000).The Mycoplasma pneumoniae infection increased the expression levels of adhesion molecules CD44 and ICAM-1 in mice.Conclusion After MP mice were cured,the expression of CD44 and ICAM-1 was down regulated,and the up regulation of CD44 and ICAM-1 expression might be related to the severity of MP pneumonia.
