The role of β-catenin deletion in Sox2-positive cells in inner-ear development and polarity regulation in mice
10.3969/j.issn.1672-8467.2018.02.001
- VernacularTitle:在Sox2阳性细胞敲除β-catenin对小鼠内耳发育和极性的影响
- Author:
Xiao-Qing QIAN
1
;
Dong-Dong REN
;
Ning CONG
;
Meng ZHAO
;
Fang-Lu CHI
Author Information
1. 复旦大学附属眼耳鼻喉科医院耳鼻喉科研究院 上海200031;复旦大学附属眼耳鼻喉科医院耳鼻喉科 上海200031;上海市听觉医学临床中心 上海200031;卫生部听觉医学重点实验室 上海200031
- Keywords:
β-catenin;
cochlea;
vestibule;
hair cell;
development;
planar cell polarity
- From:
Fudan University Journal of Medical Sciences
2018;45(2):143-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of β-catenin,the core protein of canonical Wnt/β-catenin signaling,in the inner-ear development and polarity regulation.Methods β-catenin was conditionally deleted from precursor cells of inner-ear sensory epithelium (Sox2-positive cells) in embryonic mice.Morphological changes of the inner ear were observed.Immunofluorescence staining was performed to basilar membranes,utricles and saccules to observe the hair-cell changes in arrangement and polarity.Results Compared with the control group,knock-out of β-catenin in Sox2-positive cells resulted in smaller otic vesicles,cochleas and vestibules,and fewer hair cells (HCs) in vestibular sensory epithelium (P＜0.01).Stereocilium showed misorientation and kinocillia had a change in location and quantity in the cochlea.Scattered misorented HCs were also observed in the vestibule.Conclusions β-catenin is crucial for the development of inner ear and polarity manipulation of HCs in mice.The canonical Wnt/β-catenin signaling may be involved in the regulation of cochlea extension and planar cell polarity (PCP) of inner ear,which is known to be controlled by non-canonical Wnt/PCP signaling.
