Effect of spinal cord stimulation on expression of spinal GLT-1 and GLAST in rats with diabetic neu-ropatbic pain
10.12089/jca.2018.04.017
- VernacularTitle:脊髓电刺激对糖尿病神经痛大鼠脊髓胶质细胞谷氨酸转运体-1和谷氨酸-天冬氨酸转运体的影响
- Author:
Xiang HUANG
1
;
Yating YUAN
;
Xiaohong LI
;
Shijie LI
;
Cuifen DENG
Author Information
1. 528000,佛山市第一人民医院疼痛科
- Keywords:
Spinal cord;
Electrical stimulation therapy;
Diabetic neuropathy;
Neuralgia;
Glutamate plasma membrane transport protein
- From:
The Journal of Clinical Anesthesiology
2018;34(4):386-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of spinal cord stimulation on expression of spinal GLT-1 and GLAST in rats with diabetic neuropatbic pain.Methods Forty-eight healthy male SD rats,only 2 months old,weighting 250-300 g,using the random number table method,were divided into four groups (n=12):the control group (group C),diabetes neuralgia group (group D),false stimulation group (group N)and spinal cord stimulation group (group S).The model of diabetes was induced by the pedtoneal injec-tion of streptozocin (STZ),electrodes were placed into the epidural space 1 9 days after injection of STZ in groups N and S,in addition,group S was performed 26-28 days after injection of STZ.Mechanical contrac-tion leg threshold (MWT)were determined one day before STZ injection,2 d,7 d,14 d and 28 d after STZ injection.The rats were sacrificed,the lumbar spinal cord tissue were obtained for determination of GLT-1 and GLAST expression in spinal cord tissues on 28 d after measurement of MWT.Results Compared with group C,MWT was decreased 14 d and 28 d after STZ injection,and expression levels of GLT-1 and GLAST mRNA were increased on 14 d and 28 d (P<0.05);Compared with before STZ injection,MWT of group D,group N and group S was decreased on 14 d and 28 d (P<0.05);Compared with group D, MWT was increased,and expression levels of GLT-1 and GLAST mRNA were increased in group S on 28 d after STZ injection (P<0.05 ).Conclusion The mechanism of spinal cord stimulation reducing rats diabetes neuralgia may be related to elevating the expression of GLT-1 and GLAST.
