Thyroid Papillary Carcinoma Cells TPC-1 Apoptosis Induced by Ursolic Acid
10.3969/j.issn.1003-4706.2018.01.003
- VernacularTitle:熊果酸诱导甲状腺乳头状癌细胞TPC-1凋亡的实验
- Author:
Jie HUA
1
;
Ling-Yu RUAN
;
Ding-Qian MA
;
Xiao-Shuang ZUO
;
Run-Qing XIANG
;
Shan-Dan FANG
;
Yuan FAN
Author Information
1. 云南中医学院中药学院
- Keywords:
Thyroid papillary carcinoma;
Ursolic acid;
Apoptosis;
Cycle;
Bcl-2;
Bax;
Caspase-9
- From:
Journal of Kunming Medical University
2018;39(1):10-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect of ursolic acid on the proliferation of human papillary thyroid carcinoma cell line TPC-1 in vitro. Method TPC-1 cells were treated with different concentrations of ursolic acid (control group:0μM, experimental group:3μM , 6μM, 12μM);MTT assay was used to observe the effect of the growth of TPC-1 cells on different concentrations of ursolic acid at the same time;Apoptosis and cell cycle distribution of TPC-1 cells were treated with ursolic acid by flow cytometry;The expression of Bcl-2, Bax and Caspase-9 mRNA in TPC-1 cells were treated with ursolic acid by QRT-PCR;The expression of Bcl-2, Bax and Caspase-9 protein in TPC-1 cells were treated with ursolic acid by Western blot. Results MTT assay showed that ursolic acid inhibited the proliferation of TPC-1 cells in a concentration and time-dependent manner, and the IC50 at 24 h, 48 h and 72 h was 14.21 μM, 10.56 μM, 10.39 μM; Flow cytometry showed that ursolic acid inhibited the apoptosis of TPC-1 cells in a concentration-dependent manner, and the growth of TPC-1 cells was arrested in S phase;QRT-PCR showed that Bcl-2, Bax and Caspase-9 mRNA were expressed in the control and experimental groups, ursolic acid inhibited the expression of Bcl-2 mRNA in a concentration-dependent manner and up-regulated the expression of Bax and Caspase-9 mRNA;Western blot results showed that Bcl-2, Bax and Caspase-9 were expressed in the control and experimental groups, ursolic acid inhibited the expression of Bcl-2 protein in a concentration-dependent manner and up-regulated the expression of Bax protein and Caspase-9 protein. Conclusion Ursolic acid can significantly inhibit the proliferation and induce apoptosis of human papillary thyroid TPC-1 cells, providing some ideas for the treatment of thyroid cancer.
