Nitric Oxide-Mediated Cytotoxicity of Manganese in Basal Ganglia Neuronal Cells.
- Author:
Dong Hoon SHIN
1
;
Yong Wook JUNG
;
Jae Hoon BAE
;
Dae Kyu SONG
;
Won Kyun PARK
;
Bok Hyun KO
;
Doo Hie KIM
Author Information
1. Department of Preventive Medicine, School of Medicine, Keimyung University, Korea.
- Publication Type:Original Article
- Keywords:
Manganese(Mn);
Nitric oxide(NO);
Nitric oxide synthase(NOS);
Neurotoxicity;
Neurons
- MeSH:
Animals;
Basal Ganglia*;
Cell Culture Techniques;
Equidae;
Fluorescent Antibody Technique;
Manganese*;
Microscopy, Confocal;
Neurons*;
Nitric Oxide;
Nitroarginine;
Rats;
Receptors, N-Methyl-D-Aspartate
- From:Korean Journal of Preventive Medicine
1999;32(4):459-466
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: We have investigated to manifest whether manganese-induced neurotoxicity is mediated by nitric oxide(NO) in the rat primary neuronal cultures and assess the effect of Mn2+ on the N-methyl-D aspartate(NMDA) receptors. METHODS: We have used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)assay to examine the effect of cytotoxicity of MnCl2 in neuronal cells . NO production was determined by measuring nirites, a stable oxidation product of NO. The neurons in the rat that contains neuronal nitric oxide synthase(nNOS) were examined by immunofluorescence and confocal microscopy. The effects of Mn2+ on the NMDA receptors was assesed by the whole cell voltage clamp technique. RESULTS: We showed that the NO release and NOS expression were increased with 500uM MnCl2 treatment and an NOS inhibitors, NG-nitro-L-arginine , prevented neurotoxicity elicited by manganese. In the electrophysiological study, Mn2+ does not block or activate the NMDA receptors and not pass through the NMDA receptors in a neurons of basal ganglia. CONCLUSIONS: It is concluded that manganese neurotoxicity in basal ganglia was partially mediated by nitric oxide in the cell culture model.
