Signal molecule 3-oxo-C12-HSL of Pseudomonas aeruginosa promotes autophagy of mouse alveolar macrophages MH-S cells
- VernacularTitle:铜绿假单胞菌的信号分子3-oxo-C12-HSL促进小鼠MH-S肺泡巨噬细胞自噬
- Author:
Shun-Mei E
1
;
Yang LU
;
Jian-Ming ZENG
;
Cha CHEN
Author Information
1. 广州中医药大学第二附属医院 检验医学部
- Keywords:
autophagy;
3-oxo-C12-HSL;
alveolar macrophages;
Pseudomonas aeruginosa
- From:
Basic & Clinical Medicine
2018;38(6):803-808
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 3-oxo-C12-HSL on autophagy in mouse alveolar macrophages MH-S cells. Methods MH-S cells were treated with culture supernatants of the mutant and wild type Pseudomonas aeruginosa(PA) strains of LasI gene(3-oxo-C12-HSL synthetic gene) and chemically synthesized 3-oxo-C12-HSL signaling molecules. GFP puncta was observed by laser confocal fluorescence microscopy and the ratio of LC3Ⅱ/LC3Ⅰ was detected by Western blot to detect the formation of autophagic.Autophagic flux was also detected by mo-nitoring the degradation of p62 and the change of chloroquine to LC3Ⅱ/LC3Ⅰratio. Results The supernatant of the culture medium of the wild type PA strain increased the GFP puncta of the MH-S cells(P<0.05) and the ra-tio of LC3Ⅱ/LC3Ⅰ(P<0.01),The mutant PA strain of LasI gene could not cause the above changes related to autophagy. The chemically synthesized 3-oxo-C12-HSL signal molecules could increase the number of autophagic bodies and the expression of LC3Ⅱ (P<0.01). Autophagic substrate p62 was degraded by 3-oxo-C12-HSL. Chloroquine, a lysosomal inhibitor, enhanced LC3Ⅱaccumulation caused by 3-oxo-C12-HSL (P<0.05,P<0.01).Conclusions 3-oxo-C12-HSL increases the level of autophagy in MH-S cells.
