Screening alternative splicing on long noncoding RNAs regulated by PTB in glioma
- VernacularTitle:胶质瘤中PTB调控长非编码RNA选择性剪接的筛选
- Author:
Qun-Fang WEI
1
;
Li-Yuan ZHU
;
Wei HAN
;
Xiao-Zhong PENG
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 医学分子生物学国家重点实验室生物化学与分子生物学系
- Keywords:
alternative splicing;
long noncoding RNA;
PTB;
glioma
- From:
Basic & Clinical Medicine
2018;38(6):787-792
- CountryChina
- Language:Chinese
-
Abstract:
Objective To further investigate the role of PTB in regulating the alternative splicing of lncRNAs in a glioblastoma tumorigenesis,and analyze spliced lncRNAs. Methods Analyzing array data and screening a specific set of alternative spliced lncRNAs. Total RNA was isolated from PTB knockdown glioblastoma cells (U87MG) or glioblastoma and normal cell lines and tissue samples,and subjected to real-time PCR(RT-PCR) assays to detect the expression level of spliced transcripts. Alternatively spliced lncRNAs were identified as target genes that may be regulated by PTB protein by knocking down method. Nuclear and cytoplasmic isolation were performed on T98G cells to identify cellular location of lncRNA. Results Our results uncovered PTB which impact on the transcript level of several lncRNAs including linc00882. Interestingly,the lncRNA linc00882 significantly exhibited differential spli-cing patterns between two splice variants in the PTB-abundant glioblastoma cells. The alternative splicing transcripts were located in cell cytoplasm. Conclusions The results suggest that PTB may have an effect on the alternative spli-cing of linc00882 in glioma.
