Analysis of inter-species cross-contamination in 160 non-human cell lines
- VernacularTitle:160例非人源细胞系的种间交叉污染情况分析
- Author:
Xiao-Cui BIAN
1
;
Zhen-Li YANG
;
Chun-Xia ZUO
;
Fang-Ying ZHOU
;
Yu-Qin LIU
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院病理学系
- Keywords:
non-human cell lines;
cell culture;
quality control;
species identification
- From:
Basic & Clinical Medicine
2018;38(5):632-637
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze interspecies cross-contamination of 160 non-human cell lines.Methods One hundred and sixty common non-human cell lines were collected and their species were identified by PCR.For the suspicious cells,chromosome analysis was further used to confirm their species.Results Six in 160 non-human cell lines were cross-contaminated.A rat cell line was mixed by a human cell line,and 5 were totally cross-con-taminated,and were indentified as wrong species.Conclusions Species identification is an indispensable part of cell quality control.Each cell line should undergo a full QA(Quality Assurance)assessment before it is used for research.
