Preventive effect of epigallocatechin gallate on hyperoxia-induced bronchopulmonary dysplasia in neonatal g
10.3760/cma.j.issn.1673-4408.2018.06.012
- VernacularTitle:EGCG对新生鼠高氧诱导支气管肺发育不良的预防作用
- Author:
Xiaodong LI
1
;
Haiyun HUANG
;
Shengrong ZHANG
Author Information
1. 518052,广东医科大学附属深圳南山区人民医院新生儿科
- Keywords:
Bronchopulmonary dysplasia;
Hyperoxia;
Epigallocatechin gallate;
Oxygen free radical;
Mice
- From:
International Journal of Pediatrics
2018;45(6):460-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the preventive effect of epigallocatechin gallate(EGCG)on hyperoxiainduced bronchopulmonary dysplasia in mice.Methods Eighty-eight Kunming mice born 36-40 hours were randomly divided into four groups:air + saline group (n =22),air + EGCG group (n =22),hyperoxia + saline group(n =22) and hyperoxia + EGCG group(n =22).Drug was administrated once a day by air-driven atomization,till 28 days.Furthermore,the hyperoxia groups were transferred into the room air for recovery after raised in (70 ± 3) ％ oxygen for 28 days.Lung pathological morphology and homogenization ELISA were performed on the 21th,28th and 49th day.The pathological changes of lung tissue radical alveolar counts(RAC) were observed and the levels of interleukin-2 (IL-2),tumor necrosis factor(TNF-α) and myeloperoxidase(MPO) were measured.Results The levels of IL-2 and TNF-α in the air groups were significantly lower than those in hyperoxia group(IL-2:air(1 760.83 ±303.38)pg/g vs hyperoxia(4 251.00 ±644.07) pg/g;TNF-a:air(4 308.83 ±1 114.91) pg/g vs hyperoxia(8301.83 ± 802.26) pg/g) (P ＜ 0.01),and MPO level decreased in hyperoxia +EG-CG group(7 472.83 ± 1 922)U/g,hyperoxia +NS group(4 767.68 ± 1 110.72)U/g,air + EGCG group(3712.68 ± 734.40)U/g,air + NS group(2 711.68 ± 763.39)U/g(P ＜0.05).In the hyperoxia groups,though IL-2 on 21th day and TNF-αt on 49th day were no statistic difference in hyperoxia group and air group,the inflammatory factors in EGCG group were lower than in hyperoxia + NS group.Pathology showed that the RAC count of the air groups increased gradually,while the hyperoxic groups were gradually decreased,however,the EGCG group tend to recover after 21 days recovery in room air.Conclusion Inhalation of EGCG can reduce the level of inflammatory response in lung tissue of hyperoxia exposed mice.EGCG attenuated lung injury in hyperoxia-exposed mice,and accelerated lung recovery after hyperoxia exposure in experimental animals.EGCG has potential effect to prevent hyperoxia induced bronchopulmonary dysplasia.
