RP-HPLC simultaneous determination of five isoquinoline alkaloid in Erhuang Xiaoyan tablets
10.3969/j.issn.1671-8348.2018.17.018
- VernacularTitle:反相高效液相法测定二黄消炎片中5种异喹啉类生物碱的含量
- Author:
Yanping TAN
1
;
Cailan LI
;
Lihua TAN
;
Ziren SU
Author Information
1. 广东省佛山市禅城区朝阳医院药剂科 528000
- Keywords:
chromatography,high pressure liquid;
Erhuang Xiaoyan tablets;
bererine;
epiberberine;
coptisine;
jateorhizine;
palmatine;
content determination
- From:
Chongqing Medicine
2018;47(17):2323-2326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining five isoquinoline alkaloids (including berberine,jatrorrhizine,jatrorrhizind,coptisine and palmatine) in Erhuang Xiaoyan tablets simultaneously.Methods Determination by reversed phase high performance liquid chromatography (RP-HPLC).Column:YMC-Pack ODS-AM (4.6 mm× 250.0 mm,5 μm);mobile phase:elution with 0.1％ (v/v) formic acid aqueous solution-acetonitrile mobile phase gradient;flow rate:0.4 mL/min;injection volume:10 μL;detection wavelength:345 nm;column temperature:27 ℃.Results Five of isoquinoline alkaloids in Erhuang Xiaoyan tablets were separated perfectly.The good linear relationships were obtained in the following ranges,1.526 4-5.342 4μg (r=0.999 9) for berberine,0.403 2-1.411 2 μg (r=0.999 6) for palmatine,0.309 7-1.083 9 μg (r=0.999 8) for coptisine,0.111 2-0.389 2 μg (r=0.999 8) for jatrorrhizine and 0.162 2-0.567 7 μg (r=0.999 6) for epiberberine.The recoveries were 98.69％,98.66％,98.67％,98.67％ and 98.67％,respectively.Conclusion The method possesses high feasibility,strong specificity,high accuracy and good reproducibility,which can be used for the quality control of Erhuang Xiaoyan tablets.
