Effect of sinomenine on inflammation related factors expressions in adjuvant induced arthritis model rats
10.3969/j.issn.1671-8348.2018.07.005
- VernacularTitle:青藤碱对佐剂诱导性关节炎模型大鼠炎症相关因子表达的影响
- Author:
Jing ZHAO
1
;
Weiwei LIU
;
Xueping LI
;
Yongxiang GAO
Author Information
1. 成都中医药大学基础医学院病理生理学教研室
- Keywords:
sinomenine;
rheumatoid arthritis;
interlukin-1;
interleukin-6;
interleukin-10
- From:
Chongqing Medicine
2018;47(7):879-882
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the therapeutic effect of sinomenine(SN) in adjuvant induce arthritis(AIA) model rats and its influence on inflammation related factors expressions.Methods The AIA rat model was duplicated by adopting complete Freund's adjuvant(FCA) induction method.After successfully constructing the model,the rats were randomly divided into the model group,methotrexate group,SN low,middle and high dose groups.The rats without constructing model were taken as the normal control group.Taking materials was performed after 21 d continuous medication gavage.The rat joint injury was observed by HE staining and the pathological semiquantitative scoring was performed.The serum levels of rheumatoid factor(RF),C-reactive protein (CRP),interleukin-1(IL-1),interleukin-6(IL-6),and interleukin-10(IL-10) were tested by using the automatic blood biochemical analyzer.Results Comparing with the model group,the joint injury degree in the methotrexate group,SN low and middle dose groups were obviously mild and the pathological semiquantitative score was significantly decreased(P＜0.05),serum CRP level was significantly deceased(P＜0.05),the serum inflammation related factors IL-1 and IL-6 levels were significantly decreased,while the IL-10 level was significantly increased (P＜0.01).Conclusion Low and middle dose SN can regulate the imbalance of proinflammatory factors and inflammation-suppressing factors expression levels,thus significantly improve the joint pathological injury degree in rheumatoid arthritis.
